Methods and protocols
Following are protocols and methods used in the SynBioLab. There are tweaks and updates that worked in these protocols mentioned here.
Methods and Protocols
DNA extraction from Mycobacterium tuberculosis
EMSA - Electrophoretic mobility shift assay
Preparing chemically competent E. coli
DNA Extraction from Pseudomonas aeruginosa
Preparing electrocompetent E. coli
Useful links
Calculating melting temperature of oligos – http://biotools.nubic.northwestern.edu/OligoCalc.html